bio-analytical method development and validation of rasagiline by high performance liquid chromatography tandem mass spectrometry detection and its application to pharmacokinetic study
Clicks: 241
ID: 242618
2012
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Star Article
30.0
/100
241 views
22 readers
AI Quality Assessment
Not analyzed
Readership in this journal
StarRanked #110 of 187 articles by views in Frontiers in plant science
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 187 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
The most suitable bio-analytical method based on liquidâliquid extraction has been developed and validated for quantification of Rasagiline in human plasma. Rasagiline-13C3 mesylate was used as an internal standard for Rasagiline. Zorbax Eclipse Plus C18 (2.1 mmÃ50 mm, 3.5 μm) column provided chromatographic separation of analyte followed by detection with mass spectrometry. The method involved simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode using an API-4000 system. The total run time was 3.0 min. The proposed method has been validated with the linear range of 5â12000 pg/mL for Rasagiline. The intra-run and inter-run precision values were within 1.3%â2.9% and 1.6%â2.2% respectively for Rasagiline. The overall recovery for Rasagiline and Rasagiline-13C3 mesylate analog was 96.9% and 96.7% respectively. This validated method was successfully applied to the bioequivalence and pharmacokinetic study of human volunteers under fasting condition. Keywords: High performance liquid chromatography, Mass spectrometry, Rasagiline, Liquidâliquid extraction
| Reference Key |
konda2012journalbio-analytical
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Ravi Kumar Konda;Babu Rao Chandu;B.R. Challa;Chandrasekhar B. Kothapalli |
| Journal | Frontiers in plant science |
| Year | 2012 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.