regulating the production of (r)-3-hydroxybutyrate in escherichia coli by n or p limitation

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2015
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Ranked #174 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
The chiral compound (R)-3-hydroxybutyrate (3HB) is naturally produced by many wild type organisms as the monomer for polyhydroxybutyrate (PHB). Both compounds are commercially valuable and co-polymeric polyhydroxyalkanoates have been used e.g. in medical applications for skin grafting and as components in pharmaceuticals.In this paper we investigate cultivation strategies for production of 3HB in the previously described E. coli strain AF1000 pJBGT3RX. This strain produces extracellular 3HB by expression of two genes from the PHB pathway of Halomonas boliviensis. H. boliviensis is a newly isolated halophile that forms PHB as a storage compound during carbon excess and simultaneous limitation of another nutrient like nitrogen and phosphorous. We hypothesize that a similar approach can be used to control the flux from acetyl-CoA to 3HB also in E. coli; decreasing the flux to biomass and favouring the pathway to the product. We employed ammonium- or phosphate-limited fed-batch processes for comparison of the productivity at different nutrient limitation or starvation conditions. The feed rate was shown to affect the rate of glucose consumption, respiration, 3HB and acetic acid production, although the proportions between them were more difficult to affect. The highest 3HB volumetric productivity, 1.5 g L-1 h-1, was seen for phosphate-limitation.
Referenzschlüssel
eguevara-martnez2015frontiersregulating Verwenden Sie diesen Schlüssel zum automatischen Zitieren im Manuskript bei Verwendung von SciMatic Manuscript Manager oder Thesis Manager
Autoren Mónica eGuevara-Martínez, Karin eSjöberg Gällnö, Gustav eSjöberg, Johan eJarmander, Mariel ePérez-Zabaleta, Jorge eQuillaguamán, Gen eLarsson
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Zeitschrift journal of magnetic resonance (san diego, calif : 1997)
Jahr 2015
DOI
10.3389/fmicb.2015.00844
URL
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00844/full
Schlüsselwörter
ammonium phosphate depletion e

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