cytostatic activity of geranium robertianum l. extracts processed by membrane procedures
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ID: 238109
2017
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Abstract
In the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations using Millipore membranes with 10,000 and 1000 Da cut-off. Two methods were used to establish the extracts’ antioxidant activity: DPPH and ABTS; the cytostatic capacity was evaluated on HEp-2p cell lines, by a qualitative method (cytochemical stain with Giemsa solution) and quantitative one (MTT test). Four concentrations of Geranium aqueous extracts were used to test cell viability: 100, 500, 1000 and 2000 μg/mL, 24 and 48 h, against the control culture (100% viability). The concentrated samples had the highest content of total polyphenols and the strongest antioxidant effect, having also the biggest impact upon cell viability.
| Reference Key |
neagu2017arabiancytostatic
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|---|---|
| Authors | ;Elena Neagu;Gabriela Paun;Daniel Constantin;Gabriel Lucian Radu |
| Journal | Behavioural brain research |
| Year | 2017 |
| DOI |
10.1016/j.arabjc.2013.09.028
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