crispr/cas9-mediated genome editing of mirna-155 inhibits proinflammatory cytokine production by raw264.7 cells

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ID: 237355
2015
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Abstract
MicroRNA 155 (miR-155) is a key proinflammatory regulator in clinical and experimental rheumatoid arthritis (RA). Here we generated a miR-155 genome knockout (GKO) RAW264.7 macrophage cell line using the clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CAS9) technology. While upregulating the Src homology-2 domain-containing inositol 5-phosphatase 1 (SHIP1), the miR-155 GKO line is severely impaired in producing proinflammatory cytokines but slightly increased in osteoclastogenesis upon treatment with receptor activator of nuclear factor-κB ligand (RANKL). Taken together, our results suggest that genome editing of miR-155 holds the potential as a therapeutic strategy in RA.
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jing2015biomedcrispr/cas9-mediated Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Weixia Jing, Xuewu Zhang, Wenyan Sun, Xiujuan Hou, Zhongqiang Yao, Yuelan Zhu
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Journal spectrochimica acta - part a: molecular and biomolecular spectroscopy
Year 2015
DOI
10.1155/2015/326042
URL
http://dx.doi.org/10.1155/2015/326042
Keywords
Medicine

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