sequential assessment of cell cycle s phase in flow cytometry: a non-isotopic method to measure lymphocyte activation in vitro

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ID: 237152
1997
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Abstract
Lymphocyte multiplication can be induced in vitro by mitogens or specific antigens, and is usually measured using isotopic methods involving tritiated thymidine. Cellular proliferation can also be analyzed by flow cytometry techniques based on cell cycle analysis through the measurement of DNA content. We applied this method to lymphocytes from 113 individuals, to evaluate lymphocyte proliferation after stimulation in vitro by a mitogen (phytohaemagglutinin, PHA) or a recall antigen (tetanus toxoid), using a kinetic approach with four points sequential measurements of the S and G2 phases over six days of culture. The proportion of cells in S phase after PHA stimulation was significantly higher than in controls overall and as early as on day three of the culture. Activation with a recall antigen significantly induced increasing S phase cell proportions up to day six. These data suggest that flow cytometric assessment of the S phase could be a useful alternative to isotopic methods measuring lymphocyte reactivity in vitro.
Reference Key
kohler1997analyticalsequential Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ch. Kohler;M. N. Kolopp‐Sarda;A. De March‐Kennel;A. Barbaud;M. C. Béné;G. C. Faure
Journal oriente moderno
Year 1997
DOI
10.1155/1997/376292
URL
http://dx.doi.org/10.1155/1997/376292
Keywords
neoplasms Oncology including cancer and carcinogenscytology tumors

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