two efficient techniques to find approximate overlaps between sequences

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ID: 237014
2017
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Abstract
The next-generation sequencing (NGS) technology outputs a huge number of sequences (reads) that require further processing. After applying prefiltering techniques in order to eliminate redundancy and to correct erroneous reads, an overlap-based assembler typically finds the longest exact suffix-prefix match between each ordered pair of the input reads. However, another trend has been evolving for the purpose of solving an approximate version of the overlap problem. The main benefit of this direction is the ability to skip time-consuming error-detecting techniques which are applied in the prefiltering stage. In this work, we present and compare two techniques to solve the approximate overlap problem. The first adapts a compact prefix tree to efficiently solve the approximate all-pairs suffix-prefix problem, while the other utilizes a well-known principle, namely, the pigeonhole principle, to identify a potential overlap match in order to ultimately solve the same problem. Our results show that our solution using the pigeonhole principle has better space and time consumption over an FM-based solution, while our solution based on prefix tree has the best space consumption between all three solutions. The number of mismatches (hamming distance) is used to define the approximate matching between strings in our work.
Reference Key
rachid2017biomedtwo Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Maan Haj Rachid
Journal spectrochimica acta - part a: molecular and biomolecular spectroscopy
Year 2017
DOI
10.1155/2017/2731385
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