infección de la orquídea terrestre phaius tankervilliae (orchidaceae) con el potexvirus del mosaico del cymbidium (cymmv) en costa rica
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ID: 236848
1999
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Abstract
En 1996, en un pequeño vívero comercial en la zona oeste del Valle Central de Costa Rica se observaron plantas de la orquídea terrestres Phaius tankervilliae que mostraban síntomas foliares de leves a severos de un estriado clorótico sistémico. En algunas flores los sépalos presentaron además manchas cloróticas y anillos blancos. Posteriormente en invernadero se observó que ocurría una senescencia más rápida en flores de plantas sintomáticas que en las flores de plantas sanas de la misma especie. Mediante tinción negativa de muestras purificadas se observaron al microscopio electrónico de transmisión, partículas virales filamentosas poco flexuosas, con un tamaño aproximado a 500 nm de largo y 17 nm de ancho. Al analizar las partículas virales purificadas en geles desnaturalizantes de proteínas (SDS-PAGE), se determinó la presencia de una sola proteína de cápside cercana a los 28 kDa. En geles nativos de agarosa se determinó que el virus filamentoso observado contiene un ARN con una talla aproximada a los 7 kb. Tanto el tamaño de la proteína de cápside como el del ARN de este virus purificado concuerdan con los valores informados para el grupo de los potexvirus. Mediante la prueba de ELISA se comprobó que el potexvirus purificado corresponde al virus del mosaico del Cymbidium (CymMV).
In 1996, plants of Phaius tankervilliae cultivated in a nursery in the western region of the Central Valley of Costa Rica, exhibited mild to severe foliar symptoms of chlorotic streak. Flowers from symptomatic plants were similar in size, shape and color to flowers from asymptomatic plants, but the former showed earlier senescence. In some cases, chlorosis and white rings appeared in the sepals. Virus in symptomatic leaf extracts were concentrated by ultracentrifugation and separation in sucrose gradients. Negative staining of partially purified preparations from diseased orchid plants revealed the presence of filamentous potexvirus-like particles of 500 nm in lenght and 17 nm width. Virus particles contained a single major protein of about 28 kDa as estimated by denaturalizing poli-acrylamide gel electrophoresis (SDS-PAGE) and a single RNA molecule of about 7kb. Infected plants showed a strong specific reaction in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using antiserum to cymbidium mosaic virus.
In 1996, plants of Phaius tankervilliae cultivated in a nursery in the western region of the Central Valley of Costa Rica, exhibited mild to severe foliar symptoms of chlorotic streak. Flowers from symptomatic plants were similar in size, shape and color to flowers from asymptomatic plants, but the former showed earlier senescence. In some cases, chlorosis and white rings appeared in the sepals. Virus in symptomatic leaf extracts were concentrated by ultracentrifugation and separation in sucrose gradients. Negative staining of partially purified preparations from diseased orchid plants revealed the presence of filamentous potexvirus-like particles of 500 nm in lenght and 17 nm width. Virus particles contained a single major protein of about 28 kDa as estimated by denaturalizing poli-acrylamide gel electrophoresis (SDS-PAGE) and a single RNA molecule of about 7kb. Infected plants showed a strong specific reaction in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using antiserum to cymbidium mosaic virus.
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| Authors | ;L. Moreira;W. Villalobos;E. Rodríguez-Cerezo;Carmen Rivera |
| Journal | science journal of university of zakho |
| Year | 1999 |
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