comparison of two slide agglutination serotyping methods and pcr-based capsule typing for the characterization of haemophilus influenzae serotypes comparação de dois métodos de sorotipagem de cápsula por aglutinação em lamina e o método de pcr para a caracterização de sorotipos de haemophilus influenzae

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ID: 236333
2006
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Abstract
Ninety-three nasopharyngeal Haemophilus influenzae isolates were serotyped by two slide agglutination methods (SAST 1 and SAST 2) and the results compared with those obtained by capsule type-specific PCR. SAST 1 presented a low correlation with results obtained by PCR (75.2%) while SAST 2 showed a better agreement with the molecular technique results (93.5%). These findings suggest that SAST 2 could be an alternative method for adequate detection of H.influenzae type b.
Noventa e três isolados nasofaringeanos de H. influenzae foram sorotipados através de 2 métodos de aglutinação em lamina (SAST 1 e SAST 2) e os resultados foram comparados com a sorotipagem por PCR. SAST 1 apresentou uma baixa correlação com os resultados obtidos por PCR (75,2%) enquanto que SAST 2 mostrou uma melhor concordância com os resultados da técnica molecular (93,5%). Estes resultados indicam que SAST 2 pode ser um método alternativo para a correta detecção de H. influenzae tipo b.
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silva2006braziliancomparison Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Maria E.N. Bonifácio da Silva;Paulo da Silva;Marta I.C. Medeiros;Suzel N. Neme;Claudia Macedo;José Moacir Marin
Journal ieee software
Year 2006
DOI
10.1590/S1517-83822006000100007
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100007
Keywords
Children asthma chronotherapy circadian rhythm pcr exercise training haemophilus influenzae pcrmicrobiology exercise-induced bronchospasmmedicine (general)biology (general)

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