stimulation of transient versus sustained erk1/2 phosphorylation by relative chitinase-like proteins chi3l1 and chi3l2 correlates with different kinase localization and biological outcome
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ID: 234060
2011
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Abstract
Aim. To determine 293 and U373 cell response and ERK1/2 activation profile after CHI3L1 or CHI3L2 treatment. Methods. Specific activation and localization of ERK1/2 kinases after CHI3L1 or CHI3L2 addition to unsupplemented cell medium were evaluated by Western blots, immunofluorescence and confocal microscopy. To determine whether CHI3L1 or CHI3L2 can enhance mitogenesis, [3H]thymidine incorporation in cellular DNA was measured. Results. The obtained results show that ERK1/2 phosphorylation was stimulated in both cell types (293 and U373 cells) following addition of CHI3L2 or CHI3L1 in dose- and time-dependent manner. Unexpectedly, in opposite to CHI3L1, the dose-dependent decreasing of measured mitogenesis parameters was observed in 293 and U373 cells. In both cell types the treatment with CHI3L2 gave more sustained than CHI3L1 MAPK-pathway activation with prolonged phospho-ERK1/2 nuclear accumulation in 293 cells. Conclusions. In contrast to the activation of ERK1/2 phosphorylation by CHI3L1 cell treatment, that leads to a proliferative signal, the activation of these kinases by CHI3L2 addition inhibits cell mitogenesis and proliferation in serum starved 293 and U373 cells.
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m.2011biopolymersstimulation
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| Authors | ;Kavsan V. M.;Iershov A. V.;Avdieiev S. S.;Areshkov P. O. |
| Journal | medicines |
| Year | 2011 |
| DOI |
10.7124/bc.00011F
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