molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1sl genome of aegilops longissima
Clicks: 120
ID: 233949
2015
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
120 views
22 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #58 of 63 articles by views in Chemical biology & drug design
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Wheat related diploid species Ae. longissima (2n=2x=14, SlSl) has extensive
storage protein variations that may provide useful gene resources for wheat
quality improvement. In this work, five novel 1Sl-encoded x-type high
molecular glutenin subunits (HMW-GS) were identified and designated as
1Slx-123, 1Slx-129, 1Slx1-136, 1Slx2-136 and 1Slx2.2, respectively. Their
complete open reading frames (ORFs) were cloned and sequenced by AS-PCR,
which contained 2874 bp (956 aa) for 1Slx-123, 2946 bp (979 aa) for 1Slx-129,
2901 bp (965 aa) for 1Slx1-136, 2982bp (991 aa) for 1Slx2-136 and 2928 bp
(974 aa) for 1Slx2.2. Molecular characterization demonstrated that five
unusual subunits had greater repetitive domains resulted from a larger
fragment insertion (74-113 aa). Particularly, 1Slx-129 had an extra cysteine
residue at the position 109 due to a TAT → TGT dot mutation, which may
improve the formation of superior gluten macropolymer. Our results suggest
that these unusual HMW-GS could be served as potential superior gene
resources for improving wheat gluten quality. Phylogenetic analysis revealed
that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships
with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with
those of B genome. [Projekat Ministarstva nauke Republike Srbije, br.
TR-31069]
| Reference Key |
gengrui2015genetikamolecular
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Zhu Gengrui;Wang Shunli;Zhen Shoumin;Shen Xixi;Prodanovic Slaven;Yan Yueming |
| Journal | Chemical biology & drug design |
| Year | 2015 |
| DOI |
10.2298/GENSR1501185Z
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.