produção, caracterização e aplicação de anticorpo policlonal contra azospirillum amazonense estirpe am15 production, characterization and application of polyclonal antibodies against azospirillum amazonense strain am15
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2009
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Abstract
O uso de ferramentas moleculares e imunológicas permite a detecção e o monitoramento específico de microrganismos usados como inoculantes agrícolas. O maior exemplo de sucesso com uso de bactérias diazotróficas na agricultura é o caso da inoculação de soja no Brasil. Entretanto, a inoculação de bactérias diferentes de rizóbio não permite a localização de um sítio específico onde ocorra A redução do nitrogênio atmosférico, pois não há a formação de estruturas nodulares. Este trabalho foi realizado na Embrapa-Agrobiologia, e objetivou produzir e caracterizar um anticorpo policlonal a partir da inoculação de células intactas da estirpe Am15 pertencente à espécie Azospirillum amazonense (Anti-Am15) utilizando o método de ELISA indireto. O anticorpo foi usado em sementes peletizadas com turfa contendo a bactéria-alvo e avaliada sua sobrevivência durante 60 dias. Este anticorpo foi capaz de quantificar populações bacterianas em amostras cujo número mínimo celular foi superior a valores de 100.000 células por mL. De acordo com os resultados, o soro policlonal foi especifico contra o antígeno de interesse, sendo possível sua utilização em estudos de quantificação e monitoramento do Azospirillum sp em plantas de milho que receberam inóculo e no controle da qualidade de inoculante turfoso.
Molecular and immunological approaches are useful for monitoring the localization of microrganism used as agricultural inoculants. A successful example is the use of a diazotrophic bacteria, as soybean in Brazil. However, as diazotrophic bacterias other than rizobium do not form nodular structures, the localization of the specific site where nitrogen reduction occurs is impossible. In this study, carried out at Embrapa Agrobiology, a polyclonal antibody against Azospirillum amazonense (As-Am15) was to produced and characterized through an indirect Enzyme Linked Immunosorbent Assay (ELISA). Maize seedscoated with peat containing the target bacterium were enoculated with the policlonal antibody and evaluated during 60 days. The antibody was able to quantify bacteria populations in samples where the minimal cell number were superior to 100,000 cells L-1. According to these results, the policlonal antibody As-Am15 showed high specificity to the target antigen and was able to quantify or monitor Azospirillum strains and able to be used as a control of the quality of a peat inoculant product.
Molecular and immunological approaches are useful for monitoring the localization of microrganism used as agricultural inoculants. A successful example is the use of a diazotrophic bacteria, as soybean in Brazil. However, as diazotrophic bacterias other than rizobium do not form nodular structures, the localization of the specific site where nitrogen reduction occurs is impossible. In this study, carried out at Embrapa Agrobiology, a polyclonal antibody against Azospirillum amazonense (As-Am15) was to produced and characterized through an indirect Enzyme Linked Immunosorbent Assay (ELISA). Maize seedscoated with peat containing the target bacterium were enoculated with the policlonal antibody and evaluated during 60 days. The antibody was able to quantify bacteria populations in samples where the minimal cell number were superior to 100,000 cells L-1. According to these results, the policlonal antibody As-Am15 showed high specificity to the target antigen and was able to quantify or monitor Azospirillum strains and able to be used as a control of the quality of a peat inoculant product.
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silva2009bragantiaproduo,
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| Authors | ;Marinete Flores da Silva;Veronica Massena Reis |
| Journal | revista espanola de salud publica |
| Year | 2009 |
| DOI |
10.1590/S0006-87052009000100001
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