antibodies induced by lipoarabinomannan in bovines: characterization and effects on the interaction between mycobacterium avium subsp. paratuberculosis and macrophages in vitro
Clicks: 146
ID: 230605
2011
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Popular Article
30.0
/100
146 views
6 readers
AI Quality Assessment
Not analyzed
Readership in this journal
PopularRanked #149 of 168 articles by views in Water research
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 168 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Lipoarabinomannan (LAM) is a major glycolipidic antigen on the mycobacterial envelope. The aim of this study was to characterize the humoral immune response induced by immunization with a LAM extract in bovines and to evaluate the role of the generated antibodies in the in vitro infection of macrophages with Mycobacterium avium subsp. paratuberculosis (MAP). Sera from fourteen calves immunized with LAM extract or PBS emulsified in Freund's Incomplete Adjuvant and from five paratuberculosis-infected bovines were studied. LAM-immunized calves developed specific antibodies with IgG1 as the predominant isotype. Serum immunoglobulins were isolated and their effect was examined in MAP ingestion and viability assays using a bovine macrophage cell line. Our results show that the antibodies generated by LAM immunization significantly increase MAP ingestion and reduce its intracellular viability, suggesting an active role in this model.
| Reference Key |
jolly2011veterinaryantibodies
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Ana Jolly;Silvia Beatriz Colavecchia;Bárbara Fernández;Eloy Fernández;Silvia Leonor Mundo |
| Journal | Water research |
| Year | 2011 |
| DOI |
10.4061/2011/258479
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.