quantitative histological analysis of the mandibular branch of the facial nerve in rats análise histológica quantitativa do ramo mandibular do nervo facial em ratos
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Abstract
OBJETIVO: Estabelecer um modelo para análise histológica quantitativa do ramo mandibular do nervo facial de ratos. MÉTODOS: Onze ratos Wistar tiveram os ramos mandibulares de seus nervos faciais direito e esquerdo removidos cirurgicamente, e submetidos à análise histológica quantitativa de suas regiões proximal e distal com: a) contagem total do número de axônios, b) medida da área parcial (9000μm²) de corte transversal do nervo, c) cálculo de densidade parcial (DP). RESULTADOS: Em relação à densidade dos axônios, a comparação entre os lados não mostrou diferença estatisticamente significativa (p=0,248; p=0,533). A densidade parcial média das amostras distais e proximais foi, respectivamente, 0,18 ± 0,02 e 0,19 ± 0,02 axônios/μm². A comparação entre as amostras proximais e distais não mostrou diferença estatisticamente significativa (p=0,859; p=0,182). CONCLUSÃO: Este estudo estabeleceu com sucesso um modelo de análise histológica quantitativa do ramo mandibular do nervo facial em ratos.
| Reference Key |
costa2012actaquantitative
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|---|---|
| Authors | ;Heloisa Juliana Zabeu Rossi Costa;Raquel Salomone;Ciro Ferreira da Silva;Márcio Paulino Costa;Beatriz Lucchetta Ramos;Ricardo Ferreira Bento |
| Journal | forensic science international |
| Year | 2012 |
| DOI |
10.1590/S0102-86502012001100001
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| URL | |
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