optimization of nitrogen and metal ions supplementation for very high gravity bioethanol fermentation from sweet sorghum juice using an orthogonal array design
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2012
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Abstract
Optimization of four parameters, <em>i.e.</em>, zinc (Zn<sup>2+</sup>), magnesium (Mg<sup>2+</sup>), manganese (Mn<sup>2+</sup>) and yeast extract for bioethanol production from sweet sorghum juice by <em>Saccharomyces cerevisiae </em>NP 01 under very high gravity (VHG, 270 g·L<sup>−1</sup> of total sugar) conditions was performed using an L<sub>9</sub> (3<sup>4</sup>) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 10<sup>7</sup> cells·mL<sup>−1</sup>. The results showed that the order of influence was yeast extract > Mn<sup>2+</sup> > Zn<sup>2+</sup> > Mg<sup>2+</sup> and the optimum nutrient concentrations for the ethanol fermentation were Zn<sup>2+</sup>, 0.01; Mg<sup>2+</sup>, 0.05; Mn<sup>2+</sup>, 0.04; and yeast extract, 9 g·L<sup>−1</sup>. The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration (<em>P</em>), yield (<em>Y<sub>p/s</sub></em>) and productivity (<em>Q<sub>p</sub></em>) were 120.58 ± 0.26 g·L<sup>−1</sup>, 0.49 ± 0.01 and 2.51 ± 0.01 g·L<sup>−1</sup>·h<sup>−1</sup>, respectively, while in the control treatment (without nutrient supplement) <em>P</em>, <em>Y<sub>p/s </sub></em>and <em>Q<sub>p</sub> </em>were only 93.45 ± 0.45 g·L<sup>−1</sup>, 0.49 ± 0.00 and 1.30 ± 0.01 g·L<sup>−1</sup>·h<sup>−1</sup>, respectively.
| Reference Key |
laopaiboon2012energiesoptimization
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|---|---|
| Authors | ;Lakkana Laopaiboon;Orawan Deesuth;Pattana Laopaiboon;Prasit Jaisil |
| Journal | acs combinatorial science |
| Year | 2012 |
| DOI |
10.3390/en5093178
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| URL | |
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