Discovery and Study of Novel Antihypertensive Peptides Derived from Seeds.

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ID: 22576
2019
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Abstract
Antihypertensive peptides were screened from thermolysin hydrolysate of seeds (Jue Ming Zi) using two independent bioassay-guided fractionations, reversed-phase high-performance liquid chromatography (RP-HPLC), and strong cation-exchange (SCX) liquid chromatography coupled with angiotensin I-converting enzyme (ACE) inhibitory assay. The identical peptide in the most active RP-HPLC and SCX fractions was simultaneously de novo sequenced as FHAPWK with high-resolution mass spectrometry. FHAPWK (IC = 16.83 ± 0.90 μM) was further identified as a competitive inhibitor and a true inhibitor on ACE by a Lineweaver-Burk plot and preincubation experiment, respectively. The molecular docking simulation indicated that FHAPWK could interact with several key residues of the ACE active site, which is consistent with the result of the inhibitory kinetics study. Moreover, its antihypertensive effect was demonstrated using the animal model of spontaneously hypertensive rats. It is concluded that FHAPWK is the first reported antihypertensive peptide derived from thermolysin hydrolysate of  seeds.
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shih2019discoveryjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Shih, Ya-Hui;Chen, Fu-An;Wang, Li-Fei;Hsu, Jue-Liang;
Journal Journal of agricultural and food chemistry
Year 2019
DOI
10.1021/acs.jafc.9b01922
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