Clicks: 2
ID: 224945
2013
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Abstract
We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.
| Reference Key |
higa2013memrias
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| Authors | ;Fábio Takenori Higa;Lucila Okuyama Fukasawa;Maria Gisele Gonçalves;Maristela Marques Salgado;Ana Paula Silva de Lemos;Lee H Harrison;Priscilla Lima de Oliveira;Carla Naufal da Silva;Claudio Tavares Sacchi |
| Journal | kurdistan journal of applied research |
| Year | 2013 |
| DOI |
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