Efeito do laser de baixa potência (AsGa, 904 ηm) na reparação óssea de fraturas em ratos Effect of low-level laser (GaAs, 904 ηm) for bone repair on fractures in rats
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2012
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Abstract
OBJETIVO: Analisar os efeitos do laser de baixa potência, AsGa - 904 ηm, no reparo ósseo de fraturas de tíbias em ratos. MÉTODOS: 40 ratos divididos em quatro grupos de 10 animais: grupo controle, sem fratura (GC); grupo com fratura, sem tratamento (GE II); grupo com fratura tratado com laser a 10J/cm² (GE III) e grupo com fratura tratado com laser a 15J/cm² (GE IV). A fratura foi realizada cirurgicamente e o tratamento teve duração de 45 dias, realizado em dias alternados. Finalizado o tratamento, os ratos foram submetidos à eutanásia e as tíbias tratadas foram radiografadas e submetidas a ensaios mecânicos de flexão em três pontos para avaliar a força máxima (N) para ruptura. RESULTADOS: Os valores observados de força máxima (N) foram: grupo controle (GC) de 51,5N ± 7,9N; GE II de 17,2N ± 7,8N; GE III de 16,6N ± 12,1N e GE IV de 30,3N ± 7,8N. Foram observadas diferenças estatisticamente significantes entre o grupo controle e os grupos experimentais e também entre o grupo experimental IV e os grupos experimentais II e III. Em relação às radiografias, foi observada a formação de calo ósseo em todos os grupos fraturados, indicando que passaram pelo processo normal de reparo tecidual. CONCLUSÃO: O grupo GE IV, submetido à terapia laser com dosagem de 15J/cm² obteve o maior valor para força máxima (N) entre os grupos experimentais, indicando a influência da maior dosagem do laser no reparo ósseo.
OBJECTIVE: To analyze the effects of low-level laser therapy (GaAs, 904 nm) for bone repair on tibial fractures in rats. METHODS: Forty rats were divided into four groups of 10 animals: control group without fracture (CG); fracture group without treatment (EG II); fracture group treated with laser at 10 J/cm² (EG III); and fracture group treated with laser at 15 J/cm² (EG IV). The fracture was produced surgically and the treatment lasted 45 days, done on alternate days. After treatment completion, the rats were sacrificed. The tibias were radiographed and subjected to mechanical three-point flexion tests in order to evaluate the maximum force (N) required to break them. RESULTS: The observed maximum force values (N) were: control group (CG) of 51.5 N ± 7.9 N; EG II 17.2 N ± 7.8 N; EG III 16.6 N ± 12.1 N; and EG IV 30.3 N ± 7.8 N. There were statistically significant differences between the control group and the experimental groups and also between experimental group IV and the other experimental groups (II and III). Radiographs showed callus formation in all the fractured groups, thus indicating that they had undergone the normal tissue repair process. CONCLUSION: EG IV, which underwent laser therapy with a dosage of 15 J/ cm², showed the highest maximum force value (N) among the experimental groups, thus demonstrating the influence of higher laser dosage on bone repair.
OBJECTIVE: To analyze the effects of low-level laser therapy (GaAs, 904 nm) for bone repair on tibial fractures in rats. METHODS: Forty rats were divided into four groups of 10 animals: control group without fracture (CG); fracture group without treatment (EG II); fracture group treated with laser at 10 J/cm² (EG III); and fracture group treated with laser at 15 J/cm² (EG IV). The fracture was produced surgically and the treatment lasted 45 days, done on alternate days. After treatment completion, the rats were sacrificed. The tibias were radiographed and subjected to mechanical three-point flexion tests in order to evaluate the maximum force (N) required to break them. RESULTS: The observed maximum force values (N) were: control group (CG) of 51.5 N ± 7.9 N; EG II 17.2 N ± 7.8 N; EG III 16.6 N ± 12.1 N; and EG IV 30.3 N ± 7.8 N. There were statistically significant differences between the control group and the experimental groups and also between experimental group IV and the other experimental groups (II and III). Radiographs showed callus formation in all the fractured groups, thus indicating that they had undergone the normal tissue repair process. CONCLUSION: EG IV, which underwent laser therapy with a dosage of 15 J/ cm², showed the highest maximum force value (N) among the experimental groups, thus demonstrating the influence of higher laser dosage on bone repair.
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lo2012efeitorevista
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| Authors | Léo, Jorge Alfredo;Cunha, Alessanda da;Oliveira, Elias Félix de;Prado, Reuder Pereira; |
| Journal | revista brasileira de ortopedia |
| Year | 2012 |
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