otimização de método para análise de folatos em hortaliças folhosas por cromatografia líquida de alta eficiência com detecção por fluorescência
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ID: 221063
2011
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Abstract
We optimized a method for the determination of three main folate isoforms in leafy vegetables. Analysis was carried out by HPLC, using gradient of elution and fluorescence detection. Limits of quantification were 2 ng/mL; 2 ng/mL and 28 ng/mL for tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF), respectively. Recovery rates of THF, 5-MTHF and 5-FTHF varied from 87.86 to 100.64%; 88.12-94.22% and 89.82-106.69%, respectively. Test of repeatability showed relative standard deviation for peak areas lower than 10%. Tests for linearity showed high coefficients of determination. The results showed advances in folates extraction and analysis in raw and cooked leafy vegetables.
| Reference Key |
lucia2011qumicaotimizao
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|---|---|
| Authors | ;Ceres Mattos Della Lucia;Elizangela Rodrigues da Silva;Sônia Machado Rocha Ribeiro;Helena Maria Pinheiro-Sant'Ana;Sebastião César Cardoso Brandão |
| Journal | greece and rome |
| Year | 2011 |
| DOI |
10.1590/S0100-40422011000200029
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| URL | |
| Keywords | Keywords not found |
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