generation of kcl012 research grade human embryonic stem cell line carrying a mutation in the htt gene

Clicks: 133
ID: 220027
2016
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Abstract
The KCL012 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (46 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.
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jacquet2016stemgeneration Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Laureen Jacquet;Heema Hewitson;Victoria Wood;Neli Kadeva;Glenda Cornwell;Stefano Codognotto;Carl Hobbs;Emma Stephenson;Dusko Ilic
Journal journal of energy chemistry
Year 2016
DOI
10.1016/j.scr.2016.01.012
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