efeitos metabólicos da glutamina em ratos submetidos à queimadura por água fervente (escaldadura) metabolic effects of glutamine in rats subjected to scald burn

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ID: 219466
2003
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Abstract
OBJETIVO: Investigar os efeitos metabólicos da L-glutamina (Gln) em ratos anestesiados submetidos à queimadura por água fervente. MÉTODOS: Foram estudados vinte e quatro ratos Wistar machos, anestesiados, submetidos a queimaduras da pele dorsal após exposição à água quente (100ºC) durante 10 segundos (30% de superfície corporal). Os ratos foram randomizados para receber, por gavagem, 2ml de água (G-1) ou igual volume de solução de Gln (0,5g/peso/dia) (G-2). Amostras de tecido (pele sadia e queimada, músculo e fígado) e sangue foram coletadas 24h (D1) e 48h (D2) pós-trauma para análise enzimática. RESULTADOS: A oferta de Gln induziu aumento significante nas concentrações de glicose na pele saudável em animais do G-2 no D2, e na pele queimada em G-2/D1. As concentrações de lactato também aumentaram significantemente em G-2/D1 no músculo (11,29 ± 1,25 mmol/g contra 7,43 ± 0,93 mmol/g - p<0,05) e no G-2/D2 no fígado (7,68 ± 1,49 mmol/g contra 3,27 ±0,67 mmol/g p<0,01), e em pele sadia (5,30 ± 0,42 mmol/g contra 3,57 ± 0,38 mmol/g - p<0,05). As concentrações de piruvato diminuíram significantemente no grupo G-2/D1 (músculo e fígado) e aumentaram na pele sadia no grupo G-2/D2. As concentrações de ATP diminuíram significantemente no músculo em G-2 nos tempos D1 e D2. CONCLUSÕES: A oferta de Gln sinaliza para utilização de piruvato para glicólise preponderantemente anaeróbica no músculo. O aumento nas concentrações de lactato tecidual pode vir a ser decorrente da oferta exógena de Gln, que transformada em glutamato, leva à ativação do ciclo malato-aspartato com conseqüente favorecimento da glicólise anaeróbica. A oferta de Gln induz a possível aumento na captação de glicose tanto por pele sadia quando na pele queimada. Houve falha de desenvolvimento da hipercetonemia adaptativa ao jejum em ambos os grupos. A oferta exógena de Gln parece não induzir alteração na ureagênese.
PURPOSE: Investigate the metabolic effects of L- glutamine (Gln) in rats subjected to scald burn. METHODS: Twenty-four anesthetized male Wistar rats were submitted to scald burn of dorsal skin secondary to exposure to hot water (100ºC) for 10 sec (30% of body surface). Eighteen and 42h later rats were randomized to receive (by gavage) 2ml of water (G-1) or equal volume (0,5g/Kg weight/day) of Gln solution (G-2). Tissue and blood samples were collected at the end of 24h (D1) and 48h (D2) post burn for enzymatic analysis. RESULTS: Glucose concentrations were significantly increased in healthy skin in G-2/D2 and in burned skin in G-2/D1. Lactate concentrations were significantly increased in G-2/D1 in muscle (11,29 ± 1,25 mmol/g versus 7,43 ± 0,93 mmol/g - p<0.05) and in G-2/D2 subgroups in liver (7,68 ± 1,49 mmol/g versus 3,27 ±0,67 mmol/g - p<0.01) and healthy skin (5,30 ± 0,42 mmol/g versus 3,57 ± 0,38 mmol/g - p<0.05). Pyruvate concentrations were significantly decreased in G-2/D1 subgroups in muscle and liver and increased in healthy skin in G-2/D2 subgroups. ATP concentrations were significantly decreased in muscle in G-2/D1 and G-2/D2. CONCLUSIONS: The offer of Gln signals for preponderance of pyruvate use for anaerobic glycolysis in muscle. Increase in tissue lactate concentrations may be due to exogenous offer of Gln, that once transformed in glutamate leads to the activation of malate-aspartate shuttle with consequent enhancing of anaerobic glycolysis. Offer of Gln induces possible increase in glucose uptake both in healthy and burned skin. There was a development flaw of the fast adapting hyperketonemia in both groups. Exogenous offer of Gln seems not to induce alteration in urea genesis.
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Authors ;Rômulo Cesar Costa Barbosa;Sergio Botelho Guimarães;Paulo Roberto Cavalcante de Vasconcelos;Carolyne Rolim Chaves;Paulo Roberto Leitão de Vasconcelos
Journal forensic science international
Year 2003
DOI
10.1590/S0102-86502003000600008
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502003000600008
Keywords
metabolism glutamine burns metabolismo queimaduras ratssurgery

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