single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells
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ID: 219405
2014
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Abstract
Pluripotent stem cells display significant heterogeneity in gene expression, but whether this diversity is an inherent feature of the pluripotent state remains unknown. Single-cell gene expression analysis in cell subsets defined by surface antigen expression revealed that human embryonic stem cell cultures exist as a continuum of cell states, even under defined conditions that drive self-renewal. The majority of the population expressed canonical pluripotency transcription factors and could differentiate into derivatives of all three germ layers. A minority subpopulation of cells displayed high self-renewal capacity, consistently high transcripts for all pluripotency-related genes studied, and no lineage priming. This subpopulation was characterized by its expression of a particular set of intercellular signaling molecules whose genes shared common regulatory features. Our data support a model of an inherently metastable self-renewing population that gives rise to a continuum of intermediate pluripotent states, which ultimately become primed for lineage specification.
| Reference Key |
hough2014stemsingle-cell
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|---|---|
| Authors | ;Shelley R. Hough;Matthew Thornton;Elizabeth Mason;Jessica C. Mar;Christine A. Wells;Martin F. Pera |
| Journal | nature reviews gastroenterology & hepatology |
| Year | 2014 |
| DOI |
10.1016/j.stemcr.2014.04.014
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