optimization of medium compositions for saccharomyces boulardii by box-behnken design

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ID: 218756
2016
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Abstract
Based on the previous experimental results, the Box-Behnken design (BBD) was employed to study the individual and interactive effects of sucrose, malt extract, calf serum and sodium citrate on the growth of Saccharomyces boulardii, and the optimum medium compositions were obtained. Meanwhile, the optical density (OD) in the fermentation suspension was measured at 560 nm after 36 h of incubation. It is shown that the optimized medium compositions were 36.28 g·L-1 sucrose, 6.38 g·L-1 malt extract, 5.69 g·L-1 calf serum and 5.3 g·L-1 sodium citrate. The result indicated that the growth of S. boulardii could increase significantly in the optimized medium, and the OD560nm value reached 1.397 ± 0.013 after 36 h, which increased 18.59 % compared with that of pre-optimized medium. In addition, the OD560nm value 1.397 ± 0.013 in the optimized medium was very closely to the expected value 1.394. This result suggested that optimization of medium compositions for S. boulardii by BBD in this study was reliable and effective.
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lei2016scientificoptimization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;ZHANGTENG LEI;HE CHEN;DAN HUANG;YALING ZHAI;GUOWEI SHU
Journal Mineralogical Magazine
Year 2016
DOI
DOI not found
URL
http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201604&vol=4&aid=4473
Keywords
box-behnken design saccharomyces boulardii

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