Composition and Stability of the Oxidative Phosphorylation System in the Halophile Plant .

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ID: 21782
2019
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Abstract
Mitochondria play a central role in the energy metabolism of plants. At the same time, they provide energy for plant stress responses. We here report a first view on the mitochondrial Oxidative Phosphorylation (OXPHOS) system of the halophile (salt tolerant) plant . Mitochondria were purified from suspension cultures of and for comparison of , a closely related glycophyte (salt sensitive) plant. Mitochondria were treated with digitonin and solubilized protein complexes were analyzed by 2D Blue native/SDS polyacrylamide gel electrophoresis. The OXPHOS systems of the two compared plants exhibit some distinct differences. mitochondria include a very abundant respiratory supercomplex composed of monomeric complex I and dimeric complex III. At the same time the complexes II and IV are of reduced abundance. The stability of the OXPHOS complexes was investigated by combined salt and temperature treatments of isolated mitochondria. ATP synthase (complex V) is of increased stability in . Also, the I + III supercomplex is present in high abundance during stress treatments. These results give insights into the mitochondrial contribution to the plant salt stress response.
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farhat2019compositionfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Farhat, Nèjia;Hichri, Sarra;Hildebrandt, Tatjana Manuela;Debez, Ahmed;Braun, Hans-Peter;
Journal Frontiers in plant science
Year 2019
DOI
10.3389/fpls.2019.01010
URL
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