oocytes polar body detection for automatic enucleation

Clicks: 217
ID: 217701
2016
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Abstract
Enucleation is a crucial step in cloning. In order to achieve automatic blind enucleation, we should detect the polar body of the oocyte automatically. The conventional polar body detection approaches have low success rate or low efficiency. We propose a polar body detection method based on machine learning in this paper. On one hand, the improved Histogram of Oriented Gradient (HOG) algorithm is employed to extract features of polar body images, which will increase success rate. On the other hand, a position prediction method is put forward to narrow the search range of polar body, which will improve efficiency. Experiment results show that the success rate is 96% for various types of polar bodies. Furthermore, the method is applied to an enucleation experiment and improves the degree of automatic enucleation.
Reference Key
chen2016micromachinesoocytes Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Di Chen;Mingzhu Sun;Xin Zhao
Journal chemistryopen
Year 2016
DOI
10.3390/mi7020027
URL
http://www.mdpi.com/2072-666X/7/2/27
Keywords
Machine learning micromanipulation

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