Exploration of anthelmintic activity of spp. extracts on gastrointestinal nematodes of sheep.

This study aimed to explore the phytochemical constituents and anthelmintic activities of four spp. leaves against .The extracts were prepared from four species of spp. (, , , and ). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against was done using infective larvae (L) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of spp. extract implemented were 25, 50, 100, and 200 mg/ml.Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and saponins were present in all the extracts, whereas alkaloids were absent in . . No triterpenoids were found in and . Movement of . larvae was significantly inhibited after exposure to extracts at various dosage levels ( < 0.05). The test results using LMIA on L showed the lowest inhibition in the negative control. Among the species of , the (at 200 mg/ml) showed the highest ( < 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole.Compared to other spp., exhibited the highest inhibition against L. However, the inhibition effect of was still lower as compared to albendazole.