antagonistic in vitro activity of trichoderma spp. isolates to the fungi phytophthora citrophthora /
atividade antagônica in vitro de isolados de trichoderma spp. ao fungo phytophthora citrophthora

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ID: 215655
2008
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Abstract
Gummosis is among the main fungal diseases of the citrus. It is caused by Phytophthora sp. and usually shows up in the lap of the plant, provoking rottenness and gum exudation, and expands causing the plant death for constrictions in the cambium or phloem which interrupts the descending fow of sap. The objective of this work was to evaluate the antagonistic in vitro activity of Trichoderma spp. to the fungi Phytophthora citrophthora. Phytophthora citrophthora was exposed to fve environments of antagonism (without antagonist and with four strains of Trichoderma viride, T. virens, T. harzianu and T. stromaticum). The in vitro essay was accomplished through the method of paired cultures. A completely randomized desing was used with fve treatments and three replications, and each plot was represented by three petri dishes. The isolates of Trichoderma demonstrated signifcant effect in the inhibition of the mycelial growth of the fungi Phytophthora citrophthora, and the fungi Trichoderma stromaticum presented larger antagonism to the fungi P. citrophthora while the T. harzianum presented antagonism smaller.

A citricultura nacional apresenta inúmeras pragas e doenças que limitam sua produção dentre as principais doenças fúngicas da cultura dos citros pode se destacar a gomose. A gomose causada por Phytophthora sp. geralmente se manifesta no colo da planta, provocando podridão e exsudação de goma, podendo expandir-se ocasionando a morte da planta por estrangulamento devido ao ataque do cambio ou foema, o que interrompe o fuxo descendente de seiva. Este trabalho foi realizado com o objetivo de se avaliar a atividade antagônica in vitro, de isolados de Trichoderma spp. ao fungo Phytophthora citrophthora. O fator em estudo foi um patógeno (Phytophthora citrophthora) em cinco ambientes de antagonismo (sem antagonista e com os isolados de Trichoderma viride, T. virens, T. harzianu e T. stromaticum). O ensaio in vitro foi realizado por meio do método de cultura pareada. O delineamento experimental utilizado foi inteiramente casualizado, com 5 tratamentos e 3 repetições, e cada parcela foi representada por três placas de Petri. Os isolados de Trichoderma demonstraram efeito signifcativo na inibição do crescimento micelial do fungo Phytophthora citrophthora, sendo que o fungo Trichoderma stromaticum apresentou maior antagonismo ao fungo P. citrophthora enquanto que o T. harzianum apresentou menor antagonismo.

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benett2008semina:antagonistic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Cleiton Gredson Sabin Benett;Abel Rebouças São José;Daniela Santiago Silva;Marinês Pereira Bomfim;Tiyoko Nair Hojo Rebouças;Katiane Santiago Silva
Journal frontiers in neuroendocrinology
Year 2008
DOI
10.5433/1679-0359.2008v29n4p749
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