A Liposomal Platform for Sensing of Extracellular Analytes Near Cells.

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ID: 2134
2018
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Abstract
Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca, GSH, and Zn, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca (0.25 mM⁻1 mM), Zn (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn (1 mM), Ca (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
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zhang2018abiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Xiaozhou;Heng, Sabrina;Pei, Jinxin;Morey, Jacqueline R;McDevitt, Christopher A;Abell, Andrew D;
Journal Biosensors
Year 2018
DOI
E117
URL
https://www.mdpi.com/2079-6374/8/4/117
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