comparison of the performance of disk diffusion, agar screening and e-test methods with real-time pcr for the detection of methicillin resistant coagulase negative staphylococcus strains isolated from clinical samples of shahrekord university hospitals, 2008
Clicks: 231
ID: 213197
2012
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
231 views
44 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #83 of 129 articles by views in The Journal of Chemical Physics
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 129 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Background: Coagulase Negative staphylococci (CoNS) are nosocomial pathogens. The main objective of the study was to compare the performance of disk diffusion, E-test and agar screening methods with Real-time PCR technique for detection of methicillin resistance in coagulase negative staphylococci (MRCoNS), using Taqman® Real-time PCR for mecA as the “gold standard” comparison assay. Methods: 88 coagulase negative Staphylococcus isolates were identified out of 284 Staphylococcus isolates collected from Hajar and Kashani hospitals-shahrekod, Iran. Methicillin resistance strains were identified by several methods: Disk diffusion, Agar screening, E-test and Real-time PCR. The results of the tested methods were compared with those of the Real-time PCR by Chi square or Fisher’s exact tests. Results: Of the 88 coagulase negative Staphylococcus isolates tested, 46 isolates (52.3%) were mecA-positive and 42 isolates (47.7%) were mecA-negative. The results of all the tested methods had a statistically significant agreement with those of Real-time PCR. The E-test was 100% sensitive and specific for mecA presence. The sensitivity and specificity of oxacillin agar screen (6.0 μg/ml) method were 96% and 98%, respectively and the sensitivity and specificity of oxacillin Disk diffusion method were 91% and 90%, respectively. Conclusion: In the present study, E-test is proposed as the best phenotypic method. In case economic issue matters, the oxacillin agar screening method (6.0 μg/ml), which is suitable for the detection of MRCoNS due to its accuracy and low cost, is recommended.
| Reference Key |
shariati2012iraniancomparison
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Laleh Shariati;Majid Validi;Mana Shojapour;Ali Mohammad Hasheminia;Mohammad Amin Tabatabaiefar;Ali Karimi;Mohammad Reza Nafisi |
| Journal | The Journal of Chemical Physics |
| Year | 2012 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.