the effect of laminin-1-doped nanoroughened implant surfaces: gene expression and morphological evaluation
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2012
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Abstract
Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.
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schwartz-filho2012internationalthe
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| Authors | ;Humberto Osvaldo Schwartz-Filho;Kostas Bougas;Paulo G. Coelho;Ying Xue;Mariko Hayashi;Rafael Silveira Faeda;Rosemary Adriana Chiérici Marcantonio;Daisuke Ono;Fumio Kobayashi;Kamal Mustafa;Ann Wennerberg;Ryo Jimbo |
| Journal | cities |
| Year | 2012 |
| DOI |
10.1155/2012/305638
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| URL | |
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