interaction mode between methylene blue-sm(iii) complex and herring sperm dna

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2012
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Abstract
Spectroscopic and viscosity methods were applied to investigate the interaction between methylene blue (MB)-Sm(III) complex and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.40). By means of molar ratio method, the binding ratios between MB-Sm(III) and DNA were determined as nSm(III):nMB =1:3, nMB-Sm(III) complex:n DNA = 8:1, and the apparent molar absorption coefficient of εMB-Sm(III)-DNA was found to be 7.01×105 M-1•cm-1. The bonding constants at different temperatures Kθ292K = 7.35×105 M-1, Kθ310K = 1.25×105 M-1 were obtained by double reciprocal method. Thermodynamic function computation demonstrates that ΔrSmӨ is the primary driving power of the interaction between MB-Sm(III) and herring sperm DNA. By combination analysis of the Scatchard method, circular dichroism spectroscopy and viscosity method, it suggests that the interaction mode between MB-Sm(III) complex and herring sperm DNA are a mixed binding, which contains partial intercalation and electrostatic interaction.DOI: http://dx.doi.org/10.4314/bcse.v26i3.8
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Authors Li-sheng Ding, Hai-zhuan Pan, Xing-ming Wang, Ya-min Hu
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Journal Experimental physiology
Year 2012
DOI
DOI not found
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http://www.ajol.info/index.php/bcse/article/view/80278
Keywords
complex

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