comparative effects of auxins, jasmonic acid and salicylic acid on callus initiation and organogenesis in vigna mungo (l.) hepper using hypocotyl explant

An attempt has been made to compare the effects of conventional phytohormones like IAA, NAA, BAP to the recent hormones viz., JA and SA in callus induction in blackgram using hypocotyl as explant source. Instead of testing the hormone individually, a combination of hormones was used to induce callus and organogenesis. A significant callusing response was noticed in MS medium supplemented with 0.5 ppm of 2,4-D and 1 ppm of IAA. Likewise, Salicylic acid at 1 ppm induced high percentage of callus induction proving its efficiency in inducing the root formation. Jasmonic acid induced a high percentage of callus induction at 1.5 ppm and greater rooting response than SA. Organogenic callus was observed at JA and SA supplementation. The combination of NAA, BAP and SA showed that 0.75 & 1.5 ppm of NAA, 1 ppm of BAP and 0.5 ppm of SA showed a better response in culture medium. Thus, the combination of these hormones seems serve as efficient growth supplements for in vitro culture of many agricultural crops.