development of a sphingosylphosphorylcholine detection system using rna aptamers
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ID: 210071
2010
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Abstract
Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
| Reference Key |
waga2010moleculesdevelopment
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| Authors | ;Iwao Waga;Tetsuji Tanimoto;Makio Furuichi;Asako Oka;Hiromi Masuda;Nobuya Sakai;Yuka Imai;Katsunori Horii;Kazuya Omi;Yoshihito Yoshida |
| Journal | Journal of ethnopharmacology |
| Year | 2010 |
| DOI |
10.3390/molecules15085742
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