human intersectin 2 (itsn2) binds to eps8 protein and enhances its degradation
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ID: 209476
2012
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Abstract
Participates in actin remodeling through Rac and receptor endocytosisvia Rab5. Here, we used yeast two-hybrid systemwith Eps8 as bait to screen a human brain cDNA library.ITSN2 was identified as the novel binding factor of Eps8. Theinteraction between ITSN2 and Eps8 was demonstrated by thein vivo co-immunoprecipitation and colocalization assays andthe in vitro GST pull-down assays. Furthermore, we mappedthe interaction domains to the region between amino acids260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition,protein stability assays and immunofluorescence analysisshowed ITSN2 overexpression induced the degradation ofEps8 proteins, which was markedly alleviated with the lysosomeinhibitor NH4Cl treatment. Taken together, our resultssuggested ITSN2 interacts with Eps8 and stimulates the degradationof Eps8 proteins. [BMB reports 2012; 45(3): 183-188]
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| Reference Key |
ding2012bmbhuman
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| Authors | ;Xiaofeng Ding;Zijian Yang;Fangliang Zhou;Xiang Hu;Chang Zhou;Chang Luo;Zhicheng He;Qian Liu;Hong Li;Feng Yan;Fangmei Wang;Shuanglin Xiang;Jian Zhang |
| Journal | canadian journal of physiology and pharmacology |
| Year | 2012 |
| DOI |
http://dx.doi.org/10.5483/BMBRep.2012.45.3.183
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