coupled tlc and maldi-tof/ms analyses of the lipid extract of the hyperthermophilic archaeon pyrococcus furiosus

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ID: 206989
2012
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Abstract
The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.
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lobasso2012archaeacoupled Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Simona Lobasso;Patrizia Lopalco;Roberto Angelini;Rita Vitale;Harald Huber;Volker Müller;Angela Corcelli
Journal annals of nuclear energy
Year 2012
DOI
10.1155/2012/957852
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