Back to the roots: protocol for the photoautotrophic micropropagation of medicinal .

Clicks: 268
ID: 20628
2019
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Abstract
The aim of this protocol was to develop an alternative in vitro propagation system for L. by mimicking nursery-based vegetative propagation. Photoautotrophic micropropagation (PAM) was achieved on rockwool blocks as substrate combined with commercially available fertilizer suitable for cannabis cultivation. Stock plants were initiated after sterilisation in forced-ventilated glass jars which then provided a continuous supply of shoot tip and nodal cuttings. A 97.5% rooting rate of in vitro shoot tip cuttings and successful acclimatisation were achieved within 3 weeks in glass vessels with passive ventilation.
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kodym2019backplant Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kodym, Andrea;Leeb, Christian J;
Journal plant cell, tissue and organ culture
Year 2019
DOI
10.1007/s11240-019-01635-1
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