Back to the roots: protocol for the photoautotrophic micropropagation of medicinal .
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ID: 20628
2019
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Abstract
The aim of this protocol was to develop an alternative in vitro propagation system for L. by mimicking nursery-based vegetative propagation. Photoautotrophic micropropagation (PAM) was achieved on rockwool blocks as substrate combined with commercially available fertilizer suitable for cannabis cultivation. Stock plants were initiated after sterilisation in forced-ventilated glass jars which then provided a continuous supply of shoot tip and nodal cuttings. A 97.5% rooting rate of in vitro shoot tip cuttings and successful acclimatisation were achieved within 3 weeks in glass vessels with passive ventilation.
| Reference Key |
kodym2019backplant
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|---|---|
| Authors | Kodym, Andrea;Leeb, Christian J; |
| Journal | plant cell, tissue and organ culture |
| Year | 2019 |
| DOI |
10.1007/s11240-019-01635-1
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| URL | |
| Keywords | Keywords not found |
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