studies regarding the crioprotective proprieties of the vitrification media, with glycerol, sucrose and ficoll 70 used in embryo cryopreservation
Clicks: 277
ID: 205461
2007
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Popular Article
30.0
/100
277 views
30 readers
AI Quality Assessment
Not analyzed
Readership in this journal
PopularRanked #41 of 249 articles by views in Enzyme and microbial technology
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 249 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
The aim of our paper was to make a series of experiments in order to determine theconcentration at which three cryoprotectants (glycerol, sucrose and Ficoll 70) singlyand in pairs would vitrify on plunging into liquid nitrogen and remain vitreous whenthawed in water bath. As penetrating cryoprotector we used glycerol (MW=92.09,Farmachim) and as un-penetrating cryoprotectors we used sucrose (MW=342.3,Sigma S7903) and Ficoll 70 (MW= 60,000-80,000, Sigma F4375). For glycerolthere were tested concentrations from 1M to 6.5M, with concentrations step of 0.5M.For the non penetrating cryoprotectors there were tested concentrations of 5%, 10%,15% and 20%. There were a total number of 116 solutions tested. The solutionvitrification ability on freezing was tested by plunging directly into liquid nitrogen at-196°C. There were tested three thawing temperatures 20°C, 25°C and 37°C. Theconcentration at which glycerol solution passed into vitreous state was 5M, but atthawing none of them remained vitreous at thawing. When pairs of cryoprotectorswere tested 44 solutions vitrified at freezing (23 for glycerol-sucrose, 21 forglycerol-Ficoll 70) and 11 of them remained vitreous at thawing. The glycerol-Ficoll70 pair give the best results on thawing (7 solution remained vitreous on warming)at 37°C.
| Reference Key |
telea2007scientificstudies
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;ADA TELEA;N. PĂCALĂ;ALEXANDRA IVAN;V. CARABĂ;B. KORBULY |
| Journal | Enzyme and microbial technology |
| Year | 2007 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.