Comparative Identification of MicroRNAs in Workers' Midguts in Responseto Invasion.

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ID: 20444
2019
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Abstract
Here, the expression profiles and differentially expressed miRNAs (DEmiRNAs) in the midguts of workers at 7 d and 10 d post-inoculation (dpi) with were investigated via small RNA sequencing and bioinformatics. Five hundred and twenty nine (529) known miRNAs and 25 novel miRNAs were identified in this study, and the expression of 16 predicted miRNAs was confirmed by Stem-loop RT-PCR. A total of 14 DEmiRNAs were detected in the midgut at 7 dpi, including eight up-regulated and six down-regulated miRNAs, while 12 DEmiRNAs were observed in the midgut at 10 dpi, including nine up-regulated and three down-regulated ones. Additionally, five DEmiRNAs were shared, while nine and seven DEmiRNAs were specifically expressed in midguts at 7 dpi and 10 dpi. Gene ontology analysis suggested some DEmiRNAs and corresponding target mRNAs were involved in various functions including immune system processes and response to stimulus. KEGG pathway analysis shed light on the potential functions of some DEmiRNAs in regulating target mRNAs engaged in material and energy metabolisms, cellular immunity and the humoral immune system. Further investigation demonstrated a complex regulation network between DEmiRNAs and their target mRNAs, with miR-598-y, miR-252-y, miR-92-x and miR-3654-y at the center. Our results can facilitate future exploration of the regulatory roles of miRNAs in host responses to , and provide potential candidates for further investigation of the molecular mechanisms underlying eastern honeybee-microsporidian interactions.
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chen2019comparativeinsects Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Chen, Dafu;Du, Yu;Chen, Huazhi;Fan, Yuanchan;Fan, Xiaoxue;Zhu, Zhiwei;Wang, Jie;Xiong, Cuiling;Zheng, Yanzhen;Hou, Chunsheng;Diao, Qingyun;Guo, Rui;
Journal Insects
Year 2019
DOI
E258
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