a horizontal magnetic tweezers and its use for studying single dna molecules

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ID: 203654
2018
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Abstract
We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force–extension data from our experiments show high-resolution detection of changes in the DNA tether’s extension: ~0.5 pN in the force and <10 nm change in extension. We calibrate our instrument using multiple orthogonal techniques including the well-characterized DNA overstretching transition. We also quantify the repeatability of force and extension measurements, and present data on the behavior of the overstretching transition under varying salt conditions. The design and experimental protocols are described in detail, which should enable straightforward reproduction of the tweezers.
Reference Key
fabian2018micromachinesa Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Roberto Fabian;Christopher Tyson;Pamela L. Tuma;Ian Pegg;Abhijit Sarkar
Journal chemistryopen
Year 2018
DOI
10.3390/mi9040188
URL
http://www.mdpi.com/2072-666X/9/4/188
Keywords Keywords not found

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