cytotoxic evaluation of e-liquid aerosol using different lung-derived cell models

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ID: 201266
2015
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Abstract
The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.
Reference Key
scheffler2015internationalcytotoxic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Stefanie Scheffler, Hauke Dieken, Olaf Krischenowski, Michaela Aufderheide
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Journal archives of biochemistry and biophysics
Year 2015
DOI
10.3390/ijerph121012466
URL
http://www.mdpi.com/1660-4601/12/10/12466
Keywords
Cytotoxicity electronic cigarette smoking tobacco nicotine

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