IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.

Clicks: 265
ID: 2009
2018
Article Quality & Performance Metrics
Overall Quality Improving Quality
0.0 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment
Not analyzed
Abstract
In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a "turn-on" fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed.
Reference Key
kwak2018ir783chemosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kwak, Eun-A;Kydd, LeNaiya;Lim, Butaek;Jaworski, Justyn;
Journal chemosensors
Year 2018
DOI
47
URL
Keywords
bioética; debate bioético; reproducción asistida; reproducción asistida en humanos.bioética debate bioético reproducción asistida reproducción asistida en humanos. climate change sustainable development floods natural disaster environmental design heatwave quranic translation history of quran translation urdu quran translation islamic studies evolution of quranic translations comparative quranic studies . linguistic analysis of quran digital transformation resilience circular economy green logistics firm competitiveness sustainable supply chain

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.