conservação in vitro do germoplasma de capim-elefante por meio da micropropagação de meristemas axilares in vitro conservation of elephantgrass germplasm of micropropagation of axillary meristems

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ID: 200453
1998
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Abstract
Na conservação m vitro do capim-elefante foi observado que, ao longo dos subcultivos sucessivos de meristemas picais, ocorre gradual perda de vigor nas plântulas. Essa limitação torna necessária a reintrodução de acessos na coleção, comprometendo a eficiência do processo. Visando solucionar o problema, foi conduzido um estudo com 51 cultivares de capimelefante, onde, na repicagem efetuada a cada 4 a 6 meses, foram inoculados meristemas axilares da plântula, dispostos horizontalmente sobre meio MS, adicionado ou não de ácido naftalenoacético (ANA). Neste ciclo, que durou 45 dias, foram avaliadas as seguintes variáveis: número e comprimento dos explantes, gemas brotadas por explante, início e vigor da brotação, número de brotos desenvolvidos e percentagem de morte. Os resultados sugerem que esse método melhorou o vigor acentuadamente; embora as respostas, com e sem adição de ANA ao meio, tenham sido muito variadas entre cultivares. Apenas a cv. Mercker Comum Pinda não apresentou resposta satisfatória, devendo ser futuramente submetida a outras alternativas para propagação. Com base na produção de brotos, as cvs. Napier e Sem Pêlo apresentaram o melhor desempenho, na presença de ANA. O método mostrou-se promissor também pelo uso mínimo de reguladores do crescimento, reduzindo custos e a possibilidade de mutações.
The continuous utiliwtion of explants obtainedfrom cultured apical meristems limits elephantgrass in vitro conservation because of gradual loss of vigor. Such a restriction makes it necessary to periodícally replace accessions in the coilection, reducing the efficiency of the technique. To overcome this problem, a study was carried out with 51 elephantgrass cultivars, where reculturing was conducted every 4 to 6 months using in vitro seediing axiliary meristems, with or without the addition of naphtaleneacetic acid (NAA) to the MS culture médium. During this reculturing cycle, which lasted 45 days, the following variables were evaluated: explant number and length, sprouted buds per explant, days to sprouting onset, sprouting vigor, number of developed shoots and percentage of death. The results suggest that the method improved seediing vigor markediy, altfwugh cultivar responses, regardiess of NAA addition, showed high variation. 'Mercker Comum Pinda' was the sole case showing no or weak response to either MS or NAA-enriched MS médium. Based on the number of sprouts developed, 'Napier' and 'Sem Pêlo' exhibited the best performance, when NAA was added to the médium. This method showed high potential to lower costs and reduce risks of mutations because of the limited utilization of growth regulators.
Reference Key
vidigal1998cinciaconservao Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Maria Coletta Vidigal;Leônidas Paixão Passos;José Luiz Oliveira da Silva
Journal Trials
Year 1998
DOI
10.1590/S0103-84781998000300005
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000300005
Keywords
micropropagation germplasm pennisetum purpureum micropropagação tissue cultureagricultureagriculture (general)

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