efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars
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2015
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Abstract
A set of 42 winter wheat cultivars developed from nine populations were
evaluated for distinctness. Three marker systems: morphological markers,
gliadin allele profiles and microsatellites were used to analyze distinctness
between sister cultivars. The morphological based distinctness tests for
wheat are based on a crop-specific set of characters that comply with UPOV
guidelines. The morphological markers were quite informative although they
were not capable to estimate the distances/distinctness between two pair of
sister cultivars Evropa 90 and Evropa and Novosadska rana 3 and Novosadska
rana 2. The gliadins allele profiles were the least efficient to estimate
distinctness between sister line cultivars. This system was not capable to
distinguish even cultivars developed from different populations. Sister line
wheat cultivars were fingerprinted with 19 wheat microsatellites markers. A
total of 106 alleles were detected at 19 wheat microsatellite loci, resulting
in an average allele number per marker of 5.6. The number of markers was
sufficient to distinguish among most sister line cultivars. Only one pair of
sister cultivars, Lozničanka and Kosovka, derived from the same cross could
not be distinguished. Comparing all marker systems to evaluate distinctness
of sister line wheat cultivars the most efficient was microsatellite markers
while gliadin allele profiles was the least efficient. Correlations between
matrices based on pedigree data and morphological marker, gliadin profiles
and microsatellites were significant but not large. [Projekat Ministarstva
nauke Republike Srbije, br. 31066]
| Reference Key |
srbislav2015genetikaefficiency
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|---|---|
| Authors | ;Denčić Srbislav;Depauw Ron;Momčilović Vojislava;Kondić-Špika Ankica |
| Journal | Chemical biology & drug design |
| Year | 2015 |
| DOI |
10.2298/GENSR1501219D
|
| URL | |
| Keywords |
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