purification and characterization of fibrinolytic proteases from mushroom volvariela volvaceae extract.

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ID: 199135
2010
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Abstract
Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Ediblestraw mushroom (V. volvaceae) has been known used for improvement of blood circulation due to its fibrinolyticcontent. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract.Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran(cut-off 10 kDa), and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharosecontains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specificactivity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymeshad optimum activity at temperature of 50 ºC and pH 7 in 10 minutes of incubation. Fibrin zymographic profiledemonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. Theenzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. Thissuggested that it was a serine protease. In summary, these results showed that crude and purified protease of strawmushroom (V. volvaceae) has fibrinolytic activities that can be applied for alternative thrombolytic therapy.
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praira2010makarapurification Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Willy Praira;Yanti;Irma Suparto;Dondin Sajuthi
Journal msphere
Year 2010
DOI
DOI not found
URL
http://journal.ui.ac.id/science/article/viewFile/727/691
Keywords
purification

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