fast and sensitive lc-dad-esi/ms method for analysis of saikosaponins c, a, and d from the roots of bupleurum falcatum (sandaochaihu)

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ID: 198945
2011
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Abstract
In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
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liang2011moleculesfast Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jian Liang;Yi-Nan Zheng;Yu-Fang He;Xiao-Hua Li;Zi Wang;Li-Chun Zhao;Wen-Cong Liu;Wei Li
Journal Journal of ethnopharmacology
Year 2011
DOI
10.3390/molecules16021533
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