preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type iv collagen alpha 1 chain
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2017
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Abstract
This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen.
| Reference Key |
morita2017biochemistrypreparation
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| Authors | ;Makoto Morita;Hidemitsu Sugihara;Kazuhiro Tokunaka;Arihiro Tomura;Kan Saiga;Takamichi Sato;Yasutada Imamura;Toshihiko Hayashi |
| Journal | addictive behaviors reports |
| Year | 2017 |
| DOI |
10.1016/j.bbrep.2016.11.013
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