preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type iv collagen alpha 1 chain

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2017
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Abstract
This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen.
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morita2017biochemistrypreparation Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Makoto Morita, Hidemitsu Sugihara, Kazuhiro Tokunaka, Arihiro Tomura, Kan Saiga, Takamichi Sato, Yasutada Imamura, Toshihiko Hayashi
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Journal addictive behaviors reports
Year 2017
DOI
10.1016/j.bbrep.2016.11.013
URL
http://www.sciencedirect.com/science/article/pii/S2405580816303211
Keywords
antibody type iv collagen

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