fecal specimens preparation methods for pcr diagnosis of human taeniosis
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ID: 197531
2006
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Abstract
Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.
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maroni2006revistafecal
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| Authors | ;Nunes Cáris Maroni;Lima Luiz Gustavo Ferraz;Manoel Camila Santos;Pereira Rodrigo Norberto;Nakano Mauro Massaharu;Garcia José Fernando |
| Journal | 2017 12th international conference for internet technology and secured transactions, icitst 2017 |
| Year | 2006 |
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