generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with chinese han genetic background
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2017
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Abstract
Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies.
| Reference Key |
wang2017stemgeneration
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|---|---|
| Authors | ;Ying Wang;Jing Zhang;Yue Zhang;Danyi Huang;Jian Zhao;Gang Li;Ying Lei |
| Journal | journal of energy chemistry |
| Year | 2017 |
| DOI |
10.1016/j.scr.2017.08.008
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