structural changes induced in grapevine (vitis vinifera l.) dna by femtosecond ir laser pulses: a surface-enhanced raman spectroscopic study

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ID: 196473
2016
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Ranked #85 of 133 articles by views in progress in neuro-psychopharmacology & biological psychiatry

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Abstract
In this work, surface-enhanced Raman spectra of ten genomic DNAs extracted from leaf tissues of different grapevine (Vitis vinifera L.) varieties, respectively, are analyzed in the wavenumber range 300–1800 cm−1. Furthermore, structural changes induced in grapevine genomic nucleic acids upon femtosecond (170 fs) infrared (IR) laser pulse irradiation (λ = 1100 nm) are discussed in detail for seven genomic DNAs, respectively. Surface-enhanced Raman spectroscopy (SERS) signatures, vibrational band assignments and structural characterization of genomic DNAs are reported for each case. As a general observation, the wavenumber range between 1500 and 1660 cm−1 of the spectra seems to be modified upon laser treatment. This finding could reflect changes in the base-stacking interactions in DNA. Spectral shifts are mainly attributed to purines (dA, dG) and deoxyribose. Pyrimidine residues seem to be less affected by IR femtosecond laser pulse irradiation. Furthermore, changes in the conformational properties of nucleic acid segments are observed after laser treatment. We have found that DNA isolated from Feteasca Neagra grapevine leaf tissues is the most structurally-responsive system to the femtosecond IR laser irradiation process. In addition, using unbiased computational resources by means of principal component analysis (PCA), eight different grapevine varieties were discriminated.
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dina2016nanomaterialsstructural Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Nicoleta E. Dina;Cristina M. Muntean;Nicolae Leopold;Alexandra Fălămaș;Adela Halmagyi;Ana Coste
Journal progress in neuro-psychopharmacology & biological psychiatry
Year 2016
DOI
10.3390/nano6060096
URL
http://www.mdpi.com/2079-4991/6/6/96
Keywords Keywords not found

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