deteksi dan diferensiasi virus kerdil pisang dengan teknik pcr-rflp

Clicks: 275
ID: 196439
2010
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Abstract
Banana bunchy top disease (BBTD) can be caused by the infection of two different viruses, Banana bunchy top virus (BBTV) or Abaca bunchy top virus (ABTV). Both viruses can be transmitted persistently by aphid Pentalonia nigronervosa Coq. The research was conducted to detect and to differentiate the virus bypolymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) techniques. Infected plants were collected from Yogyakarta (Sleman, Yogyakarta city, Bantul, Gunung Kidul, and KulonProgo). Nucleon Phytopure DNA Extraction Kit method was used to extract the total DNA of infected plants. Universal primers of Common DNA region (S-CRF and S-CRR) and specific primers DNA-R (C1-CRF and CI-CRR) were used for PCR amplification. PCR products were analyzed by RFLP technique using the restriction enzyme of DraI. The results reconfirm previous reports that bunchy top disease of banana in Yogyakarta is caused by BBTV. The ABTV was not detected in this present study. Based on the RFLP analysis it was concluded that BBTV collected in this study could be divided into three groups. Group 1 consisted of BBTV isolate from Sleman and Yogyakarta city with two fragments DNA of 400 and 388 bp. Group 2 consisted of isolate BBTV from Kulon Progo and Gunung Kidul with three fragments DNA of 400, 388, and 323 bp. Group 3 consisted of isolate from Bantul with two fragments DNA of 723 and 376 bp. Further study on the complete characteristics of these groups is still needed.   Penyakit kerdil pisang dapat disebabkan oleh infeksi virus yang berbeda yaitu Banana bunchy top virus(BBTV) atau Abaca bunchy top virus (ABTV). Kedua virus tersebut ditularkan secara persisten oleh kutu daun Pentalonia nigronervosa Coq. Penelitian ini bertujuan untuk mendeteksi dan membedakan virus dengan polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Tanaman terinfeksi dikoleksi dari Yogyakarta (Sleman,Kota Yogyakarta, Bantul, Gunung Kidul, dan Kulon Progo). Metode Phytopure DNA Extraction Kit dan CTAB digunakan untuk mengisolasi DNA total. Primer universal Common DNA region (S-CRF/S-CRR) dan primer spesifik DNA-R BBTV(C1-CRF danCI-CRR) digunakan dalam amplifikasi dengan PCR. Produk PCRBBTV dianalisis dengan teknik RFLP menggunakan enzim restriksi Dra I. Hasil penelitian memperkuat laporan sebelumnya bahwa penyakit kerdil pisang di Yogyakarta disebabkan oleh BBTV, dan belum ditemukan ABTV. Berdasarkan analisis RFLP, BBTV dapat dibedakan menjadi tiga kelompok. Kelompok 1 yaitu BBTV isolat Sleman dan Kota Yogyakarta dengan dua fragmen DNA berukuran sekitar 400 dan 388 bp. Kelompok 2 yaituBBTV isolat Kulon Progo dan Gunung Kidul dengan tiga fragmen DNA berukuran sekitar 400, 388, dan 323 bp. Kelompok 3 yaitu BBTV isolat Bantul dengan dua fragmen DNA berukuran sekitar 723 dan 376 bp. Penelitian tentang karakterisasi dari ketiga kelompok tersebut masih diperlukan.
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Authors ;Rahma Ayu Priani;Susamto Somowiyarjo;Sedyo Hartono;Siti Subandiyah
Journal Microorganisms
Year 2010
DOI
10.22146/jpti.11736
URL
https://jurnal.ugm.ac.id/jpti/article/view/11736
Keywords
dna extraction banana bunchy top virus pcr

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