ultra-high-performance liquid chromatography for the determination of exenatide in monkey plasma by tandem quadrupole mass spectrometry
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ID: 196350
2013
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Abstract
A highly sensitive ultra-high-performance liquid chromatographicâtandem mass spectrometry (UPLC/MS/MS) method was developed for the quantification of the synthetic peptide drug of exenatide in monkey plasma. Sample preparation was carried out by solid-phase extraction (SPE), and bivalirudin was used as the internal standard (IS). An excellent chromatographic separation was obtained on a reversed-phase C18 column with a gradient elution. Detection utilized a Qtrap 5500 system operated in the positive ion mode with multiple reaction monitoring (MRM). The proposed method was validated by assessing the specificity, linearity, intra- and inter-day precision and accuracy, recovery, and stability. The method resulted in a linear calibration range of 0.10â30ng/mL, extracting with only 50μL monkey plasma aliquots. The intra- and inter-day precisions (as relative standard deviation) were less than 7.5% and 9.6%, respectively. The method could be successfully utilized for the pharmacokinetic study of exenatide in monkeys following a single subcutaneous injection of Byetta. Keywords: Exenatide, UPLC/MS/MS, Quantitation, Pharmacokinetics, Plasma, Monkey
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zhang2013journalultra-high-performance
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| Authors | ;Jin-Feng Zhang;Chun-Jie Sha;Yu Sun;Yun-Yun Gai;Ji-Ye Sun;Jiang-Bin Han;Xin Shao;Chun-Na Sha;You-Xin Li;Wan-Hui Liu |
| Journal | Frontiers in plant science |
| Year | 2013 |
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